aav serotype 9 vectors encoding nrg4 Search Results


aav serotype 9 vectors encoding nrg4  (Genechem)
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Genechem aav serotype 9 vectors encoding nrg4
<t>Nrg4</t> deficiency accelerate the progression of osteoarthritis in mice. ( A ) Sections of knee joints were stained with HE ( n = 5). ( B ) Sections of knee joints were stained with Safranin O-fast green stains, with the magnified images highlighting the synovial tissue region ( n = 5). ( C ) OARSI scores of cartilage specimens of different groups in panel C. ( D ) OARSI scores of synovium specimens of different groups in panel C
Aav Serotype 9 Vectors Encoding Nrg4, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nrg4 deficiency accelerate the progression of osteoarthritis in mice. ( A ) Sections of knee joints were stained with HE ( n = 5). ( B ) Sections of knee joints were stained with Safranin O-fast green stains, with the magnified images highlighting the synovial tissue region ( n = 5). ( C ) OARSI scores of cartilage specimens of different groups in panel C. ( D ) OARSI scores of synovium specimens of different groups in panel C

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Neuregulin 4 inhibits synovial macrophage pro-inflammatory polarization via ErbB4/Stat5b/NF-κB signaling to alleviate osteoarthritis progression

doi: 10.1186/s13018-025-06259-0

Figure Lengend Snippet: Nrg4 deficiency accelerate the progression of osteoarthritis in mice. ( A ) Sections of knee joints were stained with HE ( n = 5). ( B ) Sections of knee joints were stained with Safranin O-fast green stains, with the magnified images highlighting the synovial tissue region ( n = 5). ( C ) OARSI scores of cartilage specimens of different groups in panel C. ( D ) OARSI scores of synovium specimens of different groups in panel C

Article Snippet: The AAV serotype 9 vectors encoding Nrg4 (GenBank accession number NM_032002.2 ) and Zsgreen were obtained from Genechem (Shanghai, China).

Techniques: Staining

Nrg4 deficiency increased the expression of synovial pro-inflammatory macrophage transformation and inflammatory cytokines in OA mice. ( A ) Sections of mice knee joints from each group were stained with F4/80 immunohistochemical staining and ( B ) the quantification of stained F4/80 positive cell ( n = 5). ( C ) The mRNA expression levels of IL-6, IL-1β, and TNF-α in the synovial tissue of mice from each group ( n = 5). ( D ) Sections of synovial tissue from the knee joints of mice from each group were stained using CD80 immunohistochemistry, and ( E ) the number of CD80-positive cells was quantified ( n = 5). ( F ) Sections of synovial tissue from the knee joints of mice from each group were stained using CD206 immunohistochemistry, and ( G ) the number of CD206-positive cells was quantified ( n = 5)

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Neuregulin 4 inhibits synovial macrophage pro-inflammatory polarization via ErbB4/Stat5b/NF-κB signaling to alleviate osteoarthritis progression

doi: 10.1186/s13018-025-06259-0

Figure Lengend Snippet: Nrg4 deficiency increased the expression of synovial pro-inflammatory macrophage transformation and inflammatory cytokines in OA mice. ( A ) Sections of mice knee joints from each group were stained with F4/80 immunohistochemical staining and ( B ) the quantification of stained F4/80 positive cell ( n = 5). ( C ) The mRNA expression levels of IL-6, IL-1β, and TNF-α in the synovial tissue of mice from each group ( n = 5). ( D ) Sections of synovial tissue from the knee joints of mice from each group were stained using CD80 immunohistochemistry, and ( E ) the number of CD80-positive cells was quantified ( n = 5). ( F ) Sections of synovial tissue from the knee joints of mice from each group were stained using CD206 immunohistochemistry, and ( G ) the number of CD206-positive cells was quantified ( n = 5)

Article Snippet: The AAV serotype 9 vectors encoding Nrg4 (GenBank accession number NM_032002.2 ) and Zsgreen were obtained from Genechem (Shanghai, China).

Techniques: Expressing, Transformation Assay, Staining, Immunohistochemical staining, Immunohistochemistry

Nrg4 re-expression inhibited synovial pro-inflammatory macrophage transformation and alleviated OA in mice. AAV-Nrg4 or AAV-Zsgreen at a dose of 1 × 10 12 viral genomes were intra-articularly injected into the joint cavity of KO mice at aged 10 weeks, followed by a standard diet for 8 weeks before sacrifice. ( A ) Nrg4 protein expression in synovial tissue ( n = 2) and ( B ) blood ( n = 5) from WT, KO, and KO mice transfected with Nrg4 [KO(AAV-Nrg4)] or Zsgreen[KO(AAV-Zsgreen)]. ( C ) Sections of mice knee joints from KO(AAV-Nrg4) and KO(AAV-Zsgreen) mice were stained with Safranin O-fast green stains ( n = 5). ( D ) OARSI scores of cartilage specimens and synovium specimens in panel C . ( E ) Sections of mice knee joints and synovial tissue from KO(AAV-Nrg4) and KO(AAV-Zsgreen) mice were subjected to immunofluorescence staining for F4/80 (green), iNOS (red), and DAPI (blue), and ( F ) the ratio of iNOS + F4/80 + cells to total F4/80 + cells was quantified ( n = 5). ( G ) Sections of mice knee joints and synovial tissue from KO(AAV-Nrg4) and KO(AAV-Zsgreen) mice were subjected to immunofluorescence staining for F4/80 (green), Arg1 (red), and DAPI (blue), and ( H ) the ratio of Arg1 + F4/80 + cells to total F4/80 + cells was quantified ( n = 5)

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Neuregulin 4 inhibits synovial macrophage pro-inflammatory polarization via ErbB4/Stat5b/NF-κB signaling to alleviate osteoarthritis progression

doi: 10.1186/s13018-025-06259-0

Figure Lengend Snippet: Nrg4 re-expression inhibited synovial pro-inflammatory macrophage transformation and alleviated OA in mice. AAV-Nrg4 or AAV-Zsgreen at a dose of 1 × 10 12 viral genomes were intra-articularly injected into the joint cavity of KO mice at aged 10 weeks, followed by a standard diet for 8 weeks before sacrifice. ( A ) Nrg4 protein expression in synovial tissue ( n = 2) and ( B ) blood ( n = 5) from WT, KO, and KO mice transfected with Nrg4 [KO(AAV-Nrg4)] or Zsgreen[KO(AAV-Zsgreen)]. ( C ) Sections of mice knee joints from KO(AAV-Nrg4) and KO(AAV-Zsgreen) mice were stained with Safranin O-fast green stains ( n = 5). ( D ) OARSI scores of cartilage specimens and synovium specimens in panel C . ( E ) Sections of mice knee joints and synovial tissue from KO(AAV-Nrg4) and KO(AAV-Zsgreen) mice were subjected to immunofluorescence staining for F4/80 (green), iNOS (red), and DAPI (blue), and ( F ) the ratio of iNOS + F4/80 + cells to total F4/80 + cells was quantified ( n = 5). ( G ) Sections of mice knee joints and synovial tissue from KO(AAV-Nrg4) and KO(AAV-Zsgreen) mice were subjected to immunofluorescence staining for F4/80 (green), Arg1 (red), and DAPI (blue), and ( H ) the ratio of Arg1 + F4/80 + cells to total F4/80 + cells was quantified ( n = 5)

Article Snippet: The AAV serotype 9 vectors encoding Nrg4 (GenBank accession number NM_032002.2 ) and Zsgreen were obtained from Genechem (Shanghai, China).

Techniques: Expressing, Transformation Assay, Injection, Transfection, Staining, Immunofluorescence

Nrg4 reduced pro-inflammatory macrophage transformation. AAV-Nrg4 or AAV-Zsgreen at a dose of 1 × 10 12 viral genomes were intra-articularly injected into the joint cavity of KO mice at aged 10 weeks, followed by a standard diet for 8 weeks before sacrifice. RAW264.7 cells were cultured at 37℃ under 5% CO 2 . For M1 macrophage polarization, cells were co-treated with 100 ng/mL LPS and 150 ng/mL rNrg4 (or vehicle control) for 24 h. For M2 macrophage polarization, cells were co-treated with 20 ng/mL IL-4 and 150 ng/mL rNrg4 (or vehicle) for 48 h. ( A ) The mRNA expression levels of IL-6, IL-1β, and TNF-α in the synovial tissue of mice from each group ( n = 5). ( B ) MTT assay for the optimum treatment condition of rNrg4 with RAW264.7 cells. The asterisk indicates the treatment conditions used in the cell experiments( n = 3 independent experiments).( C ) Representative images of M1 and M2 subtype of macrophage by flow cytometry assay and ( D ) the percentage analysis of M1 subtype in different groups ( n = 5 independent experiments). ( E ) Representative images of M1 and M2 subtype of macrophage by flow cytometry assay and ( F ) the percentage analysis of M1 subtype in different groups ( n = 5 independent experiments).( G ) Immunofluorescent staining for CD16/32 (M1 marker, red), CD206 (M2 marker, green) and DAPI (nuclei, blue). Scale bars, 5 μm. ( H ) Quantification of M1 macrophage percentage in in different groups ( n = 5 independent experiments). ( I ) Immunofluorescent staining for CD16/32 (M1 marker, red), CD206 (M2 marker, green) and DAPI (nuclei, blue). Scale bars, 5 μm. ( J ) Quantification of M2 macrophage percentage in in different groups ( n = 5 independent experiments)

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Neuregulin 4 inhibits synovial macrophage pro-inflammatory polarization via ErbB4/Stat5b/NF-κB signaling to alleviate osteoarthritis progression

doi: 10.1186/s13018-025-06259-0

Figure Lengend Snippet: Nrg4 reduced pro-inflammatory macrophage transformation. AAV-Nrg4 or AAV-Zsgreen at a dose of 1 × 10 12 viral genomes were intra-articularly injected into the joint cavity of KO mice at aged 10 weeks, followed by a standard diet for 8 weeks before sacrifice. RAW264.7 cells were cultured at 37℃ under 5% CO 2 . For M1 macrophage polarization, cells were co-treated with 100 ng/mL LPS and 150 ng/mL rNrg4 (or vehicle control) for 24 h. For M2 macrophage polarization, cells were co-treated with 20 ng/mL IL-4 and 150 ng/mL rNrg4 (or vehicle) for 48 h. ( A ) The mRNA expression levels of IL-6, IL-1β, and TNF-α in the synovial tissue of mice from each group ( n = 5). ( B ) MTT assay for the optimum treatment condition of rNrg4 with RAW264.7 cells. The asterisk indicates the treatment conditions used in the cell experiments( n = 3 independent experiments).( C ) Representative images of M1 and M2 subtype of macrophage by flow cytometry assay and ( D ) the percentage analysis of M1 subtype in different groups ( n = 5 independent experiments). ( E ) Representative images of M1 and M2 subtype of macrophage by flow cytometry assay and ( F ) the percentage analysis of M1 subtype in different groups ( n = 5 independent experiments).( G ) Immunofluorescent staining for CD16/32 (M1 marker, red), CD206 (M2 marker, green) and DAPI (nuclei, blue). Scale bars, 5 μm. ( H ) Quantification of M1 macrophage percentage in in different groups ( n = 5 independent experiments). ( I ) Immunofluorescent staining for CD16/32 (M1 marker, red), CD206 (M2 marker, green) and DAPI (nuclei, blue). Scale bars, 5 μm. ( J ) Quantification of M2 macrophage percentage in in different groups ( n = 5 independent experiments)

Article Snippet: The AAV serotype 9 vectors encoding Nrg4 (GenBank accession number NM_032002.2 ) and Zsgreen were obtained from Genechem (Shanghai, China).

Techniques: Transformation Assay, Injection, Cell Culture, Control, Expressing, MTT Assay, Flow Cytometry, Staining, Marker

ErbB4/Stat5b/NF-κB signaling involved in the regulation of Nrg4 on pro-inflammatory macrophage transformation. RAW264.7 cells were cultured at 37 °C under 5% CO2. Cells were pre-treated with 100 ng/mLLPS for 1 h, then co-treated with 150 ng/mL rNrg4 for 24 h. For STAT5b inhibition, pimozide (10 µM) was added 30 min before rNrg4. For the migration assay, RAW264.7 cells were seeded in the upper compartment of the chambers and rNrg4 for 48 h or IL-1β for 12 h was added in the lower chambers. ( A ) The mRNA levels and the concentration of TNF-α, IL-1β and IL-6 in the supernatant in RAW264.7 cells from different groups ( n = 5). ( B ) Representative migration images of macrophage. Scale bar, 100 μm. ( C ) Quantitative analysis of B ( n = 5). ( D ) GO analysis indicated that cytokine signaling in the immune system was the significantly enriched pathway. ( E ) Heat map of the stat5b and inflammation-associated genes. ( F ) The levels of Stat5b, p-Stat5b, p65, P-p65, IκBα, P -IκBα, Arg1, CD206, CD16/32, iNOS expressions in the RAW264.7 cells under different conditions (The experiment was repeated 3 times). ( G ) Quantitative analysis of p-Stat5b, P-IκBα and P-p65 in F. ( H ) The mRNA levels and the concentration of TNF-α, IL-1β and IL-6 in the supernatant in under different conditions (The experiment was repeated 3 times)

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Neuregulin 4 inhibits synovial macrophage pro-inflammatory polarization via ErbB4/Stat5b/NF-κB signaling to alleviate osteoarthritis progression

doi: 10.1186/s13018-025-06259-0

Figure Lengend Snippet: ErbB4/Stat5b/NF-κB signaling involved in the regulation of Nrg4 on pro-inflammatory macrophage transformation. RAW264.7 cells were cultured at 37 °C under 5% CO2. Cells were pre-treated with 100 ng/mLLPS for 1 h, then co-treated with 150 ng/mL rNrg4 for 24 h. For STAT5b inhibition, pimozide (10 µM) was added 30 min before rNrg4. For the migration assay, RAW264.7 cells were seeded in the upper compartment of the chambers and rNrg4 for 48 h or IL-1β for 12 h was added in the lower chambers. ( A ) The mRNA levels and the concentration of TNF-α, IL-1β and IL-6 in the supernatant in RAW264.7 cells from different groups ( n = 5). ( B ) Representative migration images of macrophage. Scale bar, 100 μm. ( C ) Quantitative analysis of B ( n = 5). ( D ) GO analysis indicated that cytokine signaling in the immune system was the significantly enriched pathway. ( E ) Heat map of the stat5b and inflammation-associated genes. ( F ) The levels of Stat5b, p-Stat5b, p65, P-p65, IκBα, P -IκBα, Arg1, CD206, CD16/32, iNOS expressions in the RAW264.7 cells under different conditions (The experiment was repeated 3 times). ( G ) Quantitative analysis of p-Stat5b, P-IκBα and P-p65 in F. ( H ) The mRNA levels and the concentration of TNF-α, IL-1β and IL-6 in the supernatant in under different conditions (The experiment was repeated 3 times)

Article Snippet: The AAV serotype 9 vectors encoding Nrg4 (GenBank accession number NM_032002.2 ) and Zsgreen were obtained from Genechem (Shanghai, China).

Techniques: Transformation Assay, Cell Culture, Inhibition, Migration, Concentration Assay

The graphical abstract. Schematic showing that Nrg4 plays a protective role in OA by alleviating the secretion of inflammatory cytokines from pro-inflammatory macrophages in the synovium

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Neuregulin 4 inhibits synovial macrophage pro-inflammatory polarization via ErbB4/Stat5b/NF-κB signaling to alleviate osteoarthritis progression

doi: 10.1186/s13018-025-06259-0

Figure Lengend Snippet: The graphical abstract. Schematic showing that Nrg4 plays a protective role in OA by alleviating the secretion of inflammatory cytokines from pro-inflammatory macrophages in the synovium

Article Snippet: The AAV serotype 9 vectors encoding Nrg4 (GenBank accession number NM_032002.2 ) and Zsgreen were obtained from Genechem (Shanghai, China).

Techniques: